3CUR
Structure of a double methionine mutant of NI-FE hydrogenase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E (A, B, C) | Periplasmic [NiFe] hydrogenase small subunit | polymer | 264 | 28347.3 | 3 | UniProt (P18187) Pfam (PF01058) Pfam (PF14720) | Desulfovibrio fructosovorans | NiFe hydrogenlyase small chain |
| 2 | B, D, F (H, I, J) | Periplasmic [NiFe] hydrogenase large subunit | polymer | 549 | 59822.4 | 3 | UniProt (P18188) Pfam (PF00374) | Desulfovibrio fructosovorans | NiFe hydrogenlyase large chain |
| 3 | BA, G, I, P, R... (C, A, B) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 6 | Chemie (SF4) | |||
| 4 | AA, H, Q (C, A, B) | FE3-S4 CLUSTER | non-polymer | 295.8 | 3 | Chemie (F3S) | |||
| 5 | CA, J, S (J, H, I) | NICKEL (II) ION | non-polymer | 58.7 | 3 | Chemie (NI) | |||
| 6 | DA, K, T (J, H, I) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 7 | EA, L, U (J, H, I) | CARBONMONOXIDE-(DICYANO) IRON | non-polymer | 135.9 | 3 | Chemie (FCO) | |||
| 8 | FA, M, V (J, H, I) | PEROXIDE ION | non-polymer | 32.0 | 3 | Chemie (PER) | |||
| 9 | GA, HA, N, O, W... (J, H, I) | GLYCEROL | non-polymer | 92.1 | 7 | Chemie (GOL) | |||
| 10 | IA, JA, KA, LA, MA... (A, H, B, I, C...) | water | water | 18.0 | 850 | Chemie (HOH) |
Sequence modifications
H, I, J: 1 - 549 (UniProt: P18188)
| PDB | External Database | Details |
|---|---|---|
| Met 74 | Val 74 | engineered mutation |
| Met 122 | Leu 122 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 264509.3 | |
| Non-Polymers* | Number of molecules | 28 |
| Total formula weight | 4394.5 | |
| All* | Total formula weight | 268903.8 |
