3CUP

Crystal structure of the MHC class II molecule I-Ag7 in complex with the peptide GAD221-235

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	H-2 class II histocompatibility antigen, A-D alpha chain	polymer	190	21623.1	1	UniProt (P04228) Pfam (PF00993) Pfam (PF07654)	Mus musculus (Mouse)
2	B (B)	MHC class II H2-IA-beta chain linked to GAD221-235 peptide	polymer	222	25539.5	1	UniProt (Q6LDA5) UniProt (Q31135) Pfam (PF00969) Pfam (PF07654) UniProt (by SIFTS) (Q05683)	Mus musculus (mouse)
3	C (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
4	D (A)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)

Sequence modifications

A: -2 - 178 (UniProt: P04228)

PDB	External Database	Details
Ser 179	-	expression tag
Ser 180	-	expression tag
Ala 181	-	expression tag
Asp 182	-	expression tag
Leu 183	-	expression tag
Val 184	-	expression tag
Pro 185	-	expression tag
Arg 186	-	expression tag

B: 221 - 235 (UniProt: Q6LDA5)

PDB	External Database	Details
Gly 215	-	expression tag
Ser 216	-	expression tag
His 217	-	expression tag
Ser 218	-	expression tag
Arg 219	-	expression tag
Gly 220	-	expression tag
Gly 236	-	linker
Ser	-	linker
Gly	-	linker
Ser	-	linker
Gly	-	linker
Ser	-	linker

B: null - 428 (UniProt: Q31135)

PDB	External Database	Details
Ser 429	-	expression tag
Ser 430	-	expression tag
Ala 431	-	expression tag
Asp 432	-	expression tag
Leu 433	-	expression tag
Val 434	-	expression tag
Pro 435	-	expression tag
Arg 436	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	47162.6
Non-Polymers*	Number of molecules	2
	Total formula weight	459.5
All*	Total formula weight	47622.1

*Water molecules are not included.