3CQ8
Ternary complex of the L415F mutant RB69 exo(-)polymerase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (T) | DNA (5'-D(*DAP*DCP*DAP*DGP*DGP*DTP*DAP*DAP*DGP*DCP*DAP*DGP*DTP*DCP*DCP*DGP*DCP*DG)-3') | polymer | 18 | 5550.6 | 1 | |||
| 2 | B (P) | DNA (5'-D(*DGP*DCP*DGP*DGP*DAP*DCP*DTP*DGP*DCP*DTP*DTP*DAP*DCP*DC)-3') | polymer | 14 | 4256.8 | 1 | |||
| 3 | C (A) | DNA polymerase | polymer | 903 | 104689.2 | 1 | UniProt (Q38087) Pfam (PF03104) Pfam (PF00136) | Enterobacteria phage | Gp43 |
| 4 | D, E, F, G, H... (A) | CALCIUM ION | non-polymer | 40.1 | 6 | Chemie (CA) | |||
| 5 | J, K, L (A) | SODIUM ION | non-polymer | 23.0 | 3 | Chemie (NA) | |||
| 6 | M (A) | THYMIDINE-5'-TRIPHOSPHATE | non-polymer | 482.2 | 1 | Chemie (TTP) | |||
| 7 | N, O, P (A) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 8 | Q, R, S (T, P, A) | water | water | 18.0 | 433 | Chemie (HOH) |
Sequence modifications
A: 1 - 903 (UniProt: Q38087)
| PDB | External Database | Details |
|---|---|---|
| Ala 222 | Asp 222 | engineered mutation |
| Ala 327 | Asp 327 | engineered mutation |
| Phe 415 | Leu 415 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 114496.5 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1067.9 | |
| All* | Total formula weight | 115564.4 |
