3CN6

Crystal structure of the Spinach Aquaporin SoPIP2;1 S274E mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Aquaporin	polymer	304	32600.8	2	UniProt (Q41372) Pfam (PF00230)	Spinacia oleracea (Spinach)
2	C, D (A, B)	CADMIUM ION	non-polymer		112.4	2	Chemie (CD)
3	E, F (A, B)	water	water		18.0	45	Chemie (HOH)

Sequence modifications

A, B: 1 - 281 (UniProt: Q41372)

PDB	External Database	Details
Met -22	-	expression tag
Ser -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Leu -11	-	expression tag
Val -10	-	expression tag
Pro -9	-	expression tag
Arg -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
His -5	-	expression tag
Met -4	-	expression tag
Leu -3	-	expression tag
Glu -2	-	expression tag
Asp -1	-	expression tag
Leu 0	-	expression tag
Glu 274	Ser 274	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	65201.6
Non-Polymers*	Number of molecules	2
	Total formula weight	224.8
All*	Total formula weight	65426.4

*Water molecules are not included.