3CIF

Crystal Structure of C153S mutant glyceraldehyde 3-phosphate dehydrogenase from Cryptosporidium parvum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Glyceraldehyde-3-phosphate dehydrogenase	polymer	359	38283.8	4	UniProt (Q7YYQ9) Pfam (PF00044) Pfam (PF02800) In PDB	Cryptosporidium parvum	GAPDH
2	A, B, C, D	GLYCERALDEHYDE-3-PHOSPHATE	non-polymer		170.1	4	Chemie (G3H)
3	A, B, C, D	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
4	B, C	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	B, D	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6		water	water		18.0	723	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 339 (UniProt: Q7YYQ9)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 153	Cys 153	engineered mutation
Leu 298	Phe 298	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	153135.0
Non-Polymers*	Number of molecules	12
	Total formula weight	3566.7
All*	Total formula weight	156701.8

*Water molecules are not included.