3CFI
Nanobody-aided structure determination of the EPSI:EPSJ pseudopilin heterdimer from Vibrio Vulnificus
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D, G, J (A, D, G, J) | Type II secretory pathway, pseudopilin EpsI | polymer | 84 | 9226.7 | 4 | UniProt (Q7MPZ1) Pfam (PF02501) | Vibrio vulnificus | |
| 2 | B, E, H, K (B, E, H, K) | Type II secretory pathway, PSEUDOPILIN EpsJ | polymer | 164 | 19203.6 | 4 | UniProt (Q7MPZ0) Pfam (PF11612) | Vibrio vulnificus | Type II secretory pathway, component EpsJ |
| 3 | C, F, I, L (C, F, I, L) | Nanobody NBEPSIJ_11 | polymer | 116 | 12614.1 | 4 | Lama glama | ||
| 4 | M, N, O, P (B, E, H, K) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 5 | AA, BA, Q, R, S... (K, L, A, B, C...) | water | water | 18.0 | 155 | Chemie (HOH) |
Sequence modifications
B, E, H, K: 31 - 194 (UniProt: Q7MPZ0)
| PDB | External Database | Details |
|---|---|---|
| Asn 86 | Ser 102 | SEE REMARK 999 |
| Thr 167 | Ala 183 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 164177.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 141.8 | |
| All* | Total formula weight | 164319.1 |
