3CBE

Development of a family of redox-sensitive green fluorescent protein indicators for use in relatively oxidizing subcellular environments

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Green fluorescent protein	polymer	250	28136.5	1	UniProt (P42212) Pfam (PF01353)	Aequorea victoria (Jellyfish)	roGFP1-iR; GFP
2	B (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
3	C (A)	water	water		18.0	239	Chemie (HOH)

Sequence modifications

A: 2 - 238 (UniProt: P42212)

PDB	External Database	Details
Met -10	-	expression tag
Arg -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Ser 48	Cys 48	engineered mutation
Cro 66	Ser 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Leu 64	Tyr 66	engineered mutation
Arg 80	Gln 80	engineered mutation
Ser 99	Phe 99	engineered mutation
Cys 147	Ser 147	engineered mutation
Arg 147	His 148	engineered mutation
Thr 153	Met 154	engineered mutation
Ala 163	Val 164	engineered mutation
Thr 167	Ile 168	engineered mutation
Cys 204	Gln 205	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28136.5
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	28160.8

*Water molecules are not included.