3C46

X-ray crystal structure of the N4 mini-vRNAP P2_7a promoter complex soaked with MgCl2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Virion RNA polymerase	polymer	1117	123036.3	2	UniProt (Q859P9) Pfam (PF21867) Pfam (PF21624) Pfam (PF21894) Pfam (PF21769)	Bacteriophage N4
2	B, D (C, D)	P2_7a Promoter DNA	polymer	36	11007.1	2
3	E, F (A, B)	DIHYDROGENPHOSPHATE ION	non-polymer		97.0	2	Chemie (2HP)
4	G, H, I, J (A, C, B, D)	water	water		18.0	967	Chemie (HOH)

Sequence modifications

A, B: 1 - 1105 (UniProt: Q859P9)

PDB	External Database	Details
Met -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Arg -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	268086.8
Non-Polymers*	Number of molecules	2
	Total formula weight	194.0
All*	Total formula weight	268280.8

*Water molecules are not included.