3C14

Complex of GS-Alpha with the Catalytic Domains of Mammalian Adenylyl Cyclase: Complex with Pyrophosphate and Ca

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Adenylate cyclase type 5	polymer	225	25526.5	1	UniProt (P30803) Pfam (PF00211) In PDB	Canis lupus familiaris (Dog)	Adenylate cyclase type V, ATP pyrophosphate-lyase 5, Adenylyl cyclase 5, Ca2+, -inhibitable adenylyl cyclase
2	B	Adenylate cyclase type 2	polymer	212	23717.0	1	UniProt (P26769) Pfam (PF00211) In PDB	Rattus norvegicus (rat)	Adenylate cyclase type II, ATP pyrophosphate-lyase 2, Adenylyl cyclase 2
3	C	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	402	46712.5	1	UniProt (P04896) Pfam (PF00503) In PDB	Bos taurus (bovine)	Adenylate cyclase-stimulating G alpha protein
4	A	FORSKOLIN	non-polymer		410.5	1	Chemie (FOK)
5	A	PYROPHOSPHATE 2-	non-polymer		176.0	1	Chemie (POP)
6	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
7	C	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
8	C	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
9	C	5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE	non-polymer		539.2	1	Chemie (GSP)
10		water	water		18.0	33	Chemie (HOH)

Sequence modifications

A: 363 - 580 (UniProt: P30803)

PDB	External Database	Details
Met 356	-	expression tag
His 357	-	expression tag
His 358	-	expression tag
His 359	-	expression tag
His 360	-	expression tag
His 361	-	expression tag
His 362	-	expression tag
Met 476	Val 557	engineered mutation

C: 1 - 394 (UniProt: P04896)

PDB	External Database	Details
Gly 395	-	expression tag
Gly 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	95956.0
Non-Polymers*	Number of molecules	6
	Total formula weight	1225.5
All*	Total formula weight	97181.6

*Water molecules are not included.