3BW4

Crystal structures and site-directed mutagenesis study of nitroalkane oxidase from Streptomyces ansochromogenes

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	2-nitropropane dioxygenase	polymer	369	38521.3	1	UniProt (Q9FDD4) Pfam (PF03060) In PDB	Streptomyces ansochromogenes
2	A	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	1	Chemie (FMN)
3	A	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
4		water	water		18.0	136	Chemie (HOH)

Sequence modifications

A: 1 - 363 (UniProt: Q9FDD4)

PDB	External Database	Details
Tyr 320	Trp 320	engineered mutation
His 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	38521.3
Non-Polymers*	Number of molecules	2
	Total formula weight	574.5
All*	Total formula weight	39095.8

*Water molecules are not included.