3BVV
Golgi mannosidase II D204A catalytic nucleophile mutant complex with METHYL ALPHA-D-MANNOPYRANOSYL-(1->3)-[6-THIO-ALPHA-D-MANNOPYRANOSYL-(1->6)]-BETA-D-MANNOPYRANOSIDE
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Alpha-mannosidase 2 | polymer | 1045 | 119657.6 | 1 | UniProt (Q24451) Pfam (PF01074) Pfam (PF09261) Pfam (PF21260) Pfam (PF07748) Pfam (PF18230) In PDB | Drosophila melanogaster (Fruit fly) | Alpha-mannosidase II, Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase, MAN II, Golgi alpha-mannosidase II, AMAN II |
2 | B | alpha-D-mannopyranose-(1-3)-[6-thio-alpha-D-mannopyranose-(1-6)]methyl beta-D-mannopyranoside | branched | 534.5 | 1 | In PDB GlyTouCan (G40399NH) | |||
3 | A | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
4 | A | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
5 | A | (4R)-2-METHYLPENTANE-2,4-DIOL | non-polymer | 118.2 | 1 | Chemie (MRD) | |||
6 | A | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 2 | Chemie (MPD) | |||
7 | water | water | 18.0 | 1657 | Chemie (HOH) |
Sequence modifications
A: 13 - 1045 (UniProt: Q24451)
PDB | External Database | Details |
---|---|---|
Arg 1 | - | expression tag |
Ser 2 | - | expression tag |
Ser 3 | - | expression tag |
His 4 | - | expression tag |
His 5 | - | expression tag |
His 6 | - | expression tag |
His 7 | - | expression tag |
His 8 | - | expression tag |
His 9 | - | expression tag |
Gly 10 | - | expression tag |
Glu 11 | - | expression tag |
Phe 12 | - | expression tag |
Ala 204 | Asp 267 | engineered mutation |
Lys 907 | Glu 970 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 119657.6 | |
Branched | Number of molecules | 1 |
Total formula weight | 534.5 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 514.9 | |
All* | Total formula weight | 120707.0 |