3BUQ

Golgi alpha-mannosidase II D204A catalytic nucleophile mutant with bound mannose.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Alpha-mannosidase 2	polymer	1045	119657.6	1	UniProt (Q24451) Pfam (PF01074) Pfam (PF09261) Pfam (PF21260) Pfam (PF07748) Pfam (PF18230)	Drosophila melanogaster (Fruit fly)	Alpha-mannosidase II, Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase, MAN II, Golgi alpha-mannosidase II, AMAN II
2	B (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
3	C (A)	alpha-D-mannopyranose	non-polymer		180.2	1	Chemie (MAN)
4	D (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	E (A)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
6	F (A)	water	water		18.0	1034	Chemie (HOH)

Sequence modifications

A: 13 - 1045 (UniProt: Q24451)

PDB	External Database	Details
Arg 1	-	expression tag
Ser 2	-	expression tag
Ser 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
Gly 10	-	expression tag
Glu 11	-	expression tag
Phe 12	-	expression tag
Ala 204	Asp 267	engineered mutation
Lys 907	Glu 970	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	119657.6
Non-Polymers*	Number of molecules	4
	Total formula weight	584.9
All*	Total formula weight	120242.5

*Water molecules are not included.