3BUD

Golgi mannosidase II D204A catalytic nucleophile mutant with an empty active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Alpha-mannosidase 2	polymer	1045	119657.6	1	UniProt (Q24451) Pfam (PF01074) Pfam (PF09261) Pfam (PF21260) Pfam (PF07748) Pfam (PF18230)	Drosophila melanogaster (Fruit fly)	Alpha-mannosidase II, Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase, MAN II, Golgi alpha-mannosidase II, AMAN II
2	B (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
3	C (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
4	D (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	E, F, G (A)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	3	Chemie (MPD)
6	H (A)	water	water		18.0	1498	Chemie (HOH)

Sequence modifications

A: 13 - 1045 (UniProt: Q24451)

PDB	External Database	Details
Arg 1	-	expression tag
Ser 2	-	expression tag
Ser 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
Gly 10	-	expression tag
Glu 11	-	expression tag
Phe 12	-	expression tag
Ala 204	Asp 267	engineered mutation
Lys 907	Glu 970	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	119657.6
Non-Polymers*	Number of molecules	6
	Total formula weight	736.1
All*	Total formula weight	120393.7

*Water molecules are not included.