3BPL

Crystal structure of the IL4-IL4R-Common Gamma ternary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Interleukin-4	polymer	129	14989.2	1	UniProt (P05112) Pfam (PF00727)	Homo sapiens (human)	IL-4, B-cell stimulatory factor 1, BSF-1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra
2	B (B)	Interleukin-4 receptor alpha chain	polymer	205	23470.3	1	UniProt (P24394) Pfam (PF09238)	Homo sapiens (human)	IL-4R-alpha, CD124 antigen, Soluble interleukin-4 receptor alpha chain
3	C (C)	Cytokine receptor common gamma chain	polymer	199	23912.7	1	UniProt (P31785) Pfam (PF22012) Pfam (PF21605)	Homo sapiens (human)	Gamma-C, Interleukin-2 receptor gamma chain, IL-2R gamma chain, p64, CD132 antigen
4	D (D)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
5	E (E)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
6	F, G, H (C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
7	I, J, K (A, B, C)	water	water		18.0	137	Chemie (HOH)

Sequence modifications

B: 2 - 202 (UniProt: P24394)

PDB	External Database	Details
Ala -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Phe 1	-	expression tag
Gln 28	Asn 53	engineered mutation
Gln 73	Asn 98	engineered mutation
Gln 109	Asn 134	engineered mutation
Gln 151	Asn 176	engineered mutation

C: 34 - 232 (UniProt: P31785)

PDB	External Database	Details
Gln 53	Asn 75	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	62372.3
Branched	Number of molecules	2
	Total formula weight	994.9
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	64030.8

*Water molecules are not included.