3BF8

1.1 resolution structure of ybfF, a new esterase from Escherichia coli: a unique substrate-binding crevice generated by domain arrangement

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Esterase YbfF	polymer	255	28602.4	2	UniProt (J7QD06) Pfam (PF12697) UniProt (by SIFTS) (P75736)	Escherichia coli
2	C, D (A, B)	MALONIC ACID	non-polymer		104.1	2	Chemie (MLA)
3	E, F (A, B)	water	water		18.0	825	Chemie (HOH)

Sequence modifications

A, B: 1 - 254 (UniProt: J7QD06)

PDB	External Database	Details
Val 50	Met 50	engineered mutation
Gln 78	Leu 78	engineered mutation
His 255	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57204.7
Non-Polymers*	Number of molecules	2
	Total formula weight	208.1
All*	Total formula weight	57412.9

*Water molecules are not included.