3BDG

Crystal structure of wild-type/T155V mixed dimer of E. coli alkaline phosphatase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Alkaline phosphatase	polymer	458	48320.8	1	UniProt (P00634) Pfam (PF00245)	Escherichia coli	APase
2	B (B)	Alkaline phosphatase	polymer	458	48268.5	1	UniProt (P00634) Pfam (PF00245)	Escherichia coli	APase
3	C, D, E, F (A, B)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
4	G, H (A, B)	water	water		18.0	1257	Chemie (HOH)

Sequence modifications

A: 0 - 449 (UniProt: P00634)

PDB	External Database	Details
Val 155	Thr 177	engineered mutation
Leu 450	-	expression tag
Glu 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
His 455	-	expression tag
His 456	-	expression tag
His 457	-	expression tag

B: 0 - 449 (UniProt: P00634)

PDB	External Database	Details
Leu 450	-	expression tag
Glu 451	-	expression tag
Glu 452	-	expression tag
Glu 453	-	expression tag
Glu 454	-	expression tag
Glu 455	-	expression tag
Glu 456	-	expression tag
Glu 457	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	96589.3
Non-Polymers*	Number of molecules	4
	Total formula weight	384.3
All*	Total formula weight	96973.6

*Water molecules are not included.