3BDF
Crystal structure of metal-free E. coli alkaline phosphatase (T155V)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Alkaline phosphatase | polymer | 458 | 48320.8 | 2 | UniProt (P00634) Pfam (PF00245) | Escherichia coli | APase |
| 2 | C, D, E, F (A, B) | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 3 | G, H (A, B) | water | water | 18.0 | 1222 | Chemie (HOH) |
Sequence modifications
A, B: 0 - 449 (UniProt: P00634)
| PDB | External Database | Details |
|---|---|---|
| Val 155 | Thr 177 | engineered mutation |
| Leu 450 | - | expression tag |
| Glu 451 | - | expression tag |
| His 452 | - | expression tag |
| His 453 | - | expression tag |
| His 454 | - | expression tag |
| His 455 | - | expression tag |
| His 456 | - | expression tag |
| His 457 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 96641.5 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 384.3 | |
| All* | Total formula weight | 97025.8 |
