3B8U

Crystal structure of Escherichia coli alaine racemase mutant E221A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Alanine racemase	polymer	379	41358.2	4	UniProt (P0A6B4) Pfam (PF01168) Pfam (PF00842)	Escherichia coli
2	E, F, G, I, J... (A, B, C, D)	SULFATE ION	non-polymer		96.1	12	Chemie (SO4)
3	H, L, P, T (A, B, C, D)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	4	Chemie (PLP)
4	U, V, W, X (A, B, C, D)	water	water		18.0	354	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 359 (UniProt: P0A6B4)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 221	Glu 221	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	165432.8
Non-Polymers*	Number of molecules	16
	Total formula weight	2141.3
All*	Total formula weight	167574.1

*Water molecules are not included.