3B0M
M175K mutant of assimilatory nitrite reductase (Nii3) from tobbaco leaf
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Nitrite reductase | polymer | 584 | 65569.0 | 1 | UniProt (Q76KB0) Pfam (PF03460) Pfam (PF01077) | Nicotiana tabacum (tobacco) | NII3 |
| 2 | B (A) | SIROHEME | non-polymer | 916.7 | 1 | Chemie (SRM) | |||
| 3 | C (A) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 1 | Chemie (SF4) | |||
| 4 | D (A) | POTASSIUM ION | non-polymer | 39.1 | 1 | Chemie (K) | |||
| 5 | E, F (A) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 6 | G (A) | water | water | 18.0 | 682 | Chemie (HOH) |
Sequence modifications
A: -6 - 555 (UniProt: Q76KB0)
| PDB | External Database | Details |
|---|---|---|
| Met -28 | - | expression tag |
| Gly -27 | - | expression tag |
| His -26 | - | expression tag |
| His -25 | - | expression tag |
| His -24 | - | expression tag |
| His -23 | - | expression tag |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| Ser -16 | - | expression tag |
| Ser -15 | - | expression tag |
| Gly -14 | - | expression tag |
| His -13 | - | expression tag |
| Ile -12 | - | expression tag |
| Glu -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Arg -9 | - | expression tag |
| His -8 | - | expression tag |
| Met -7 | - | expression tag |
| Lys 175 | Met 200 | engineered mutation |
| Arg 290 | Lys 315 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 65569.0 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1378.3 | |
| All* | Total formula weight | 66947.3 |
