3AXE
The truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase V18Y/W203Y in complex with cellotetraose (cellobiose density was observed)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Beta-glucanase | polymer | 249 | 27827.7 | 1 | UniProt (P17989) Pfam (PF00722) In PDB | Fibrobacter succinogenes | 1,3-1,4-beta-D-glucan 4-glucanohydrolase, Endo-beta-1,3-1,4 glucanase, Lichenase, Mixed linkage beta-glucanase |
| 2 | B | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900005) GlyTouCan (G84824ZO) | beta-cellobiose | ||
| 3 | A | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 4 | A | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 5 | water | water | 18.0 | 332 | Chemie (HOH) |
Sequence modifications
A: 2 - 248 (UniProt: P17989)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | expression tag |
| Met 4 | Ala 27 | engineered mutation |
| Tyr 18 | Val 41 | engineered mutation |
| Ser 65 | Asn 88 | engineered mutation |
| Tyr 203 | Trp 226 | engineered mutation |
| Leu 249 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 27827.7 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 342.3 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 282.5 | |
| All* | Total formula weight | 28452.4 |
