3AO0

Crystal structure of ethanolamine ammonia-lyase from Escherichia coli complexed with CN-CBL and (S)-2-amino-1-propanol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Ethanolamine ammonia-lyase heavy chain	polymer	453	49447.8	2	UniProt (P0AEJ6) Pfam (PF06751)	Escherichia coli	Ethanolamine ammonia-lyase large subunit
2	B, D (B, D)	Ethanolamine ammonia-lyase light chain	polymer	263	28769.0	2	UniProt (P19636) Pfam (PF05985)	Escherichia coli	Ethanolamine ammonia-lyase small subunit
3	E, G (A, C)	(2S)-2-aminopropan-1-ol	non-polymer		75.1	2	Chemie (2A1)
4	F, I (B, D)	COBALAMIN	non-polymer		1330.4	2	Chemie (B12)
5	H (C)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
6	J, K, L, M (A, B, C, D)	water	water		18.0	592	Chemie (HOH)

Sequence modifications

B, D: 44 - 295 (UniProt: P19636)

PDB	External Database	Details
Met 33	-	expression tag
Asp 34	-	expression tag
Gln 35	-	expression tag
Ser 36	-	expression tag
Ser 37	-	expression tag
His 38	-	expression tag
His 39	-	expression tag
His 40	-	expression tag
His 41	-	expression tag
His 42	-	expression tag
His 43	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	156433.7
Non-Polymers*	Number of molecules	5
	Total formula weight	2833.9
All*	Total formula weight	159267.6

*Water molecules are not included.