3AMP

E134C-Cellotetraose complex of cellulase 12A from thermotoga maritima

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Endo-1,4-beta-glucanase	polymer	265	30753.6	2	UniProt (Q60032) Pfam (PF01670) In PDB	Thermotoga maritima	cellulase 12A
2	C, E	beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		504.4	2	In PDB BIRD (PRD_900014) GlyTouCan (G03861VK)		alpha-cellotriose
3	D, F	beta-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900005) GlyTouCan (G84824ZO)		beta-cellobiose
4		water	water		18.0	536	Chemie (HOH)

Sequence modifications

A, B: 1 - 257 (UniProt: Q60032)

PDB	External Database	Details
Met -7	-	expression tag
Gly -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Cys 134	Glu 134	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61507.3
Branched	Number of molecules	4
	Total formula weight	1693.5
All*	Total formula weight	63200.7

*Water molecules are not included.