3AIM
R267E mutant of a HSL-like carboxylesterase from Sulfolobus tokodaii
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | 303aa long hypothetical esterase | polymer | 323 | 35984.9 | 4 | UniProt (Q976W8) Pfam (PF07859) In PDB | Sulfolobus tokodaii | Carboxylesterase |
2 | A, B | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 3 | Chemie (MPD) | |||
3 | A, B, C, D | PHOSPHATE ION | non-polymer | 95.0 | 5 | Chemie (PO4) | |||
4 | B, C, D | (4R)-2-METHYLPENTANE-2,4-DIOL | non-polymer | 118.2 | 7 | Chemie (MRD) | |||
5 | water | water | 18.0 | 158 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 303 (UniProt: Q976W8)
PDB | External Database | Details |
---|---|---|
Met -19 | - | expression tag |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Glu 267 | Arg 267 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 143939.5 | |
Non-Polymers* | Number of molecules | 15 |
Total formula weight | 1656.6 | |
All* | Total formula weight | 145596.1 |