3AI9

Crystal structure of DUF358 protein reveals a putative SPOUT-class rRNA methyltransferase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (X)	UPF0217 protein MJ1640	polymer	211	24647.5	1	UniProt (Q59034) Pfam (PF04013)	Methanocaldococcus jannaschii (Methanococcus jannaschii)
2	B (X)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
3	C, D (X)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	E (X)	water	water		18.0	174	Chemie (HOH)

Sequence modifications

X: 1 - 205 (UniProt: Q59034)

PDB	External Database	Details
Gly 1	-	expression tag
Ser 1	-	expression tag
His 1	-	expression tag
Mse 1	-	expression tag
Ala 1	-	expression tag
Ser 1	-	expression tag
Mse 190	Ile 190	engineered mutation
Mse 192	Ile 192	engineered mutation
Mse 193	Ile 193	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	24647.5
Non-Polymers*	Number of molecules	3
	Total formula weight	447.0
All*	Total formula weight	25094.5

*Water molecules are not included.