3AHV

Semi-active E176Q mutant of rice bglu1 covalent complex with 2-deoxy-2-fluoroglucoside

Replaces:  3F5I

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Beta-glucosidase 7	polymer	481	54727.5	2	UniProt (Q75I93) Pfam (PF00232) In PDB	Oryza sativa Japonica Group (Japanese rice)	Os3bglu7
2	A, B	2-deoxy-2-fluoro-alpha-D-glucopyranose	non-polymer		182.1	2	Chemie (G2F)
3	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	A, B	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5	A, B	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
6	A, B	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
7		water	water		18.0	662	Chemie (HOH)

Sequence modifications

A, B: 1 - 476 (UniProt: Q75I93)

PDB	External Database	Details
Ala -4	-	expression tag
Met -3	-	expression tag
Ala -2	-	expression tag
Asp -1	-	expression tag
Val 0	-	expression tag
Val 24	Ala 52	SEE REMARK 999
Gln 176	Glu 204	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	109455.0
Non-Polymers*	Number of molecules	11
	Total formula weight	1380.7
All*	Total formula weight	110835.6

*Water molecules are not included.