3AHJ

H553A mutant of Phosphoketolase from Bifidobacterium Breve

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Xylulose 5-phosphate/fructose 6-phosphate phosphoketolase	polymer	845	94895.8	1	UniProt (D6PAH1) Pfam (PF09364) Pfam (PF03894) Pfam (PF09363) In PDB	Bifidobacterium breve	PHOSPHOKETOLASE
2	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
3	A	THIAMINE DIPHOSPHATE	non-polymer		425.3	1	Chemie (TPP)
4	A	1,2-ETHANEDIOL	non-polymer		62.1	11	Chemie (EDO)
5		water	water		18.0	542	Chemie (HOH)

Sequence modifications

A: 1 - 825 (UniProt: D6PAH1)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 553	His 553	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	94895.8
Non-Polymers*	Number of molecules	13
	Total formula weight	1132.4
All*	Total formula weight	96028.2

*Water molecules are not included.