3AHH

H142A mutant of Phosphoketolase from Bifidobacterium Breve complexed with acetyl thiamine diphosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Xylulose 5-phosphate/fructose 6-phosphate phosphoketolase	polymer	845	94895.8	1	UniProt (D6PAH1) Pfam (PF09364) Pfam (PF03894) Pfam (PF09363) In PDB	Bifidobacterium breve	PHOSPHOKETOLASE
2	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
3	A	2-ACETYL-THIAMINE DIPHOSPHATE	non-polymer		467.4	1	Chemie (HTL)
4	A	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5	A	1,2-ETHANEDIOL	non-polymer		62.1	8	Chemie (EDO)
6	A	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
7		water	water		18.0	553	Chemie (HOH)

Sequence modifications

A: 1 - 825 (UniProt: D6PAH1)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 142	His 142	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	94895.8
Non-Polymers*	Number of molecules	13
	Total formula weight	1228.4
All*	Total formula weight	96124.2

*Water molecules are not included.