3AEU

Structure of the light-independent protochlorophyllide reductase catalyzing a key reduction for greening in the dark

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Light-independent protochlorophyllide reductase subunit N	polymer	436	47212.1	2	UniProt (P26164) Pfam (PF00148)	Rhodobacter capsulatus	LI-POR subunit N, DPOR subunit N, dark-operative protochlorophyllide oxidoreductase N-protein
2	B, D (B, D)	Light-independent protochlorophyllide reductase subunit B	polymer	525	57217.5	2	UniProt (P26163) Pfam (PF00148) Pfam (PF08369)	Rhodobacter capsulatus	LI-POR subunit B, DPOR subunit B, dark-operative protochlorophyllide oxidoreductase B-protein
3	E, F (A, C)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
4	G, H, I, J (A, B, C, D)	water	water		18.0	15	Chemie (HOH)

Sequence modifications

A, C: 2 - 424 (UniProt: P26164)

PDB	External Database	Details
Met -11	-	expression tag
Ala -10	-	expression tag
Ser -9	-	expression tag
Trp -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
Pro -5	-	expression tag
Gln -4	-	expression tag
Phe -3	-	expression tag
Glu -2	-	expression tag
Lys -1	-	expression tag
Gly 0	-	expression tag
Ala 1	-	expression tag

B, D: 1 - 525 (UniProt: P26163)

PDB	External Database	Details
Ala 36	Asp 36	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	208859.2
Non-Polymers*	Number of molecules	2
	Total formula weight	703.3
All*	Total formula weight	209562.5

*Water molecules are not included.