3AEN

Reaction intermediate structure of Entamoeba histolytica methionine gamma-lyase 1 containing Michaelis complex and alpha-amino-alpha, beta-butenoic acid-pyridoxal-5'-phosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Methionine gamma-lyase	polymer	389	42578.9	2	UniProt (Q86D28) Pfam (PF01053) In PDB	Entamoeba histolytica
2	B, D	Methionine gamma-lyase	polymer	389	42350.8	2	UniProt (Q86D28) Pfam (PF01053) In PDB	Entamoeba histolytica
3	A	METHIONINE	non-polymer		149.2	1	Chemie (MET)
4	A, B, C, D	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
5	A, C	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6	B, D	(2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid	non-polymer		330.2	2	Chemie (4LM)
7		water	water		18.0	874	Chemie (HOH)

Sequence modifications

A, C: 1 - 389 (UniProt: Q86D28)

PDB	External Database	Details
Leu 308	Ser 308	SEE REMARK 999

B, D: 501 - 889 (UniProt: Q86D28)

PDB	External Database	Details
Leu 808	Ser 308	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	169859.4
Non-Polymers*	Number of molecules	10
	Total formula weight	1474.2
All*	Total formula weight	171333.5

*Water molecules are not included.