3A66
Crystal structure of 6-aminohexanoate-dimer hydrolase S112A/G181D/H266N/D370Y mutant with substrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 6-AMINOHEXANOATE-DIMER HYDROLASE | polymer | 392 | 42905.6 | 1 | UniProt (P07061) UniProt (P07062) Pfam (PF00144) | Flavobacterium | NYLON OLIGOMERS-DEGRADING ENZYME EII, NYLON OLIGOMERS-DEGRADING ENZYME EII' |
| 2 | B, C (A) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 3 | D, E, F, G, H... (A) | GLYCEROL | non-polymer | 92.1 | 7 | Chemie (GOL) | |||
| 4 | K, L (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 2 | Chemie (MES) | |||
| 5 | M (A) | 6-AMINOHEXANOIC ACID | non-polymer | 131.2 | 1 | Chemie (ACA) | |||
| 6 | N (A) | water | water | 18.0 | 400 | Chemie (HOH) |
Sequence modifications
A: 1 - 21 (UniProt: P07061)
A: 22 - 392 (UniProt: P07062)
A: 22 - 392 (UniProt: P07062)
| PDB | External Database | Details |
|---|---|---|
| Ala 112 | Ser 112 | engineered mutation |
| Asp 181 | Gly 181 | engineered mutation |
| Arg 190 | Ala 190 | SEE REMARK 999 |
| His 191 | Thr 191 | SEE REMARK 999 |
| Asn 266 | His 266 | engineered mutation |
| Tyr 370 | Asp 370 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 42905.6 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 1212.3 | |
| All* | Total formula weight | 44117.9 |
