3A5N
Crystal Structure of a Dictyostelium P109A Ca2+-Actin in Complex with Human Gelsolin Segment 1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (S) | Gelsolin | polymer | 127 | 14205.0 | 1 | UniProt (P06396) Pfam (PF00626) | HOMO SAPIENS (human) | Actin-depolymerizing factor, ADF, Brevin, AGEL |
| 2 | B (C) | Major actin | polymer | 375 | 41432.2 | 1 | UniProt (P07830) Pfam (PF00022) | DICTYOSTELIUM DISCOIDEUM (SLIME MOLD) | Actin-1, Actin-2, Actin-2-sub 1, Actin-4, Actin-5, Actin-6, Actin-7, Actin-8, Actin A8, Actin-IEL1, Actin-9, Actin-11, Actin-12, Actin A12, Actin-13, Actin-14, Actin-15, Actin A1, Actin III, Actin-3a, Actin-16, Actin M6, Actin-19, Actin-20, Actin-21 |
| 3 | C, D, E (S, C) | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
| 4 | F (C) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 5 | G, H (S, C) | water | water | 18.0 | 368 | Chemie (HOH) |
Sequence modifications
S: 4 - 127 (UniProt: P06396)
C: 1 - 375 (UniProt: P07830)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Gly 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Cys 35 | Asn 84 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 109 | Pro 110 | engineered mutation |
| Ala 205 | Glu 206 | engineered mutation |
| Ala 206 | Arg 207 | engineered mutation |
| Ala 207 | Glu 208 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 55637.2 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 627.4 | |
| All* | Total formula weight | 56264.6 |
