39NW
The crystal structure of a solute-binding protein (L70F and N280D mutant) from Trichormus variabilis ATCC 29413 in complex with isoleucine.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Amino acid/amide ABC transporter substrate-binding protein, HAAT family | polymer | 395 | 42398.4 | 2 | UniProt (Q3MFZ5) | Trichormus variabilis ATCC 29413 | |
| 2 | C, H (A, B) | ISOLEUCINE | non-polymer | 131.2 | 2 | Chemie (ILE) PubChem (6306) | |||
| 3 | D, I (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 4 | E, F (A) | FORMIC ACID | non-polymer | 46.0 | 2 | Chemie (FMT) PubChem (284) | |||
| 5 | G, J, K (A, B) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) PubChem (753) | |||
| 6 | L, M (A, B) | water | water | 18.0 | 488 | Chemie (HOH) |
Sequence modifications
A, B: 25 - 416 (UniProt: Q3MFZ5)
| PDB | External Database | Details |
|---|---|---|
| Ser 22 | - | expression tag |
| Asn 23 | - | expression tag |
| Ala 24 | - | expression tag |
| Phe 70 | Leu 70 | engineered mutation |
| Asp 280 | Asn 280 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 84796.8 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 679.3 | |
| All* | Total formula weight | 85476.1 |
