38RK

Cryo-EM structure of the L274A mutant of the Arabidopsis thaliana mechanosensitive channel of small conductance-like protein 2 (MSL2) in DOPC lipid nanodiscs.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Mechanosensitive ion channel protein 2, chloroplastic,Green fluorescent protein	polymer	866	95799.1	7	UniProt (Q56X46) UniProt (P42212)	Arabidopsis thaliana (thale cress)	Mechanosensitive channel of small conductance-like 2,MscS-Like protein 2

Sequence modifications

A, B, C, D, E, F, G: 76 - 673 (UniProt: Q56X46)

PDB	External Database	Details
Met 75	-	initiating methionine
Ala 274	Leu 274	engineered mutation
Ser 674	-	linker
Asn 675	-	linker
Ser 676	-	linker
Leu 677	-	linker
Glu 678	-	linker
Val 679	-	linker
Leu 680	-	linker
Phe 681	-	linker
Gln 682	-	linker
Gly 683	-	linker
Pro 684	-	linker
Thr 685	-	linker
Ala 686	-	linker
Ala 687	-	linker
Ala 688	-	linker
Ala 689	-	linker
Val 690	-	linker

A, B, C, D, E, F, G: 691 - 927 (UniProt: P42212)

PDB	External Database	Details
Leu 753	Phe 64	conflict
Thr 754	Ser 65	conflict
Lys 895	Ala 206	conflict
Leu 920	His 231	conflict
Ser 928	-	expression tag
Gly 929	-	expression tag
Gly 930	-	expression tag
Gly 931	-	expression tag
Thr 932	-	expression tag
Glu 933	-	expression tag
Thr 934	-	expression tag
Ser 935	-	expression tag
Gln 936	-	expression tag
Val 937	-	expression tag
Ala 938	-	expression tag
Pro 939	-	expression tag
Ala 940	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	670593.9
All*	Total formula weight	670593.9

*Water molecules are not included.