38EX

Isoreticular co-crystal 1 with symmetrical expanded duplex (31mer) containing insert sequence CCCGGCCGGA with poly-T tail

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Replication initiation protein	polymer	263	30749.1	1	UniProt (P03856)	Escherichia coli	Protein E,Protein rep,Protein F4, Replication Initiator protein RepE54
2	B (B)	DNA (31-MER)	polymer	31	9459.1	1		Escherichia coli
3	C (C)	DNA (5'-D(P*GP*CP*CP*GP*GP*GP*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*TP*T)-3')	polymer	21	6510.2	1		Escherichia coli
4	D (D)	DNA (5'-D(*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*TP*CP*CP*G)-3')	polymer	15	4576.0	1		Escherichia coli
5	E (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)

Sequence modifications

A: 1 - 251 (UniProt: P03856)

PDB	External Database	Details
Met -11	-	initiating methionine
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Pro 118	Arg 118	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	51294.3
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	51318.6

*Water molecules are not included.