38EW
Isoreticular co-crystal 1 with symmetrical expanded duplex (31mer) containing insert sequence CCCGGCCGGA with poly-C tail and poly-G guest
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA (31-MER) | polymer | 31 | 9459.1 | 1 | Escherichia coli | ||
| 2 | B (B) | DNA (5'-D(P*CP*CP*GP*GP*GP*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*CP*C)-3') | polymer | 17 | 5238.4 | 1 | Escherichia coli | ||
| 3 | C (C) | Replication initiation protein | polymer | 263 | 30515.7 | 1 | UniProt (P03856) | Escherichia coli | Protein E,Protein rep,Protein F4 |
| 4 | D (D) | DNA (5'-D(P*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*TP*CP*CP*GP*G)-3') | polymer | 16 | 4905.2 | 1 | Escherichia coli | ||
| 5 | E (C) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) |
Sequence modifications
C: 1 - 251 (UniProt: P03856)
| PDB | External Database | Details |
|---|---|---|
| Met -11 | - | initiating methionine |
| Arg -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Ser -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 53 | Leu 53 | conflict |
| Gly 54 | Gln 54 | conflict |
| Gly 55 | Glu 55 | conflict |
| Pro 118 | Arg 118 | conflict |
| Glu 172 | Tyr 172 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 50118.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 24.3 | |
| All* | Total formula weight | 50142.7 |
