38EP

Isoreticular co-crystal 1 with asymmetrical expanded duplex containing insert sequence GACGGCCCGG and Y184E mutation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA (31-MER)	polymer	31	9507.1	1		Escherichia coli
2	B (B)	DNA (31-MER)	polymer	31	9545.1	1		Escherichia coli
3	C (C)	Replication initiation protein	polymer	263	30515.7	1	UniProt (P03856)	Escherichia coli	Protein E,Protein rep,Protein F4
4	D (C)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)

Sequence modifications

C: 1 - 251 (UniProt: P03856)

PDB	External Database	Details
Met -11	-	initiating methionine
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 53	Leu 53	conflict
Gly 54	Gln 54	conflict
Gly 55	Glu 55	conflict
Pro 118	Arg 118	conflict
Glu 172	Tyr 172	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	49568.0
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	49592.3

*Water molecules are not included.