37XT
Polynucleotide kinase phosphatase bound to a nucleosome containing a nick with a 3'-phosphate and 5'-OH at SHL-4 (FHA, composite)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | Histone H3.2 | polymer | 135 | 15257.8 | 2 | UniProt (Q71DI3) | Homo sapiens (human) | H3-clustered histone 13,H3-clustered histone 14,H3-clustered histone 15,Histone H3/m,Histone H3/o |
| 2 | B, F (B, F) | Histone H4 | polymer | 102 | 11263.2 | 2 | UniProt (P62805) | Homo sapiens (human) | |
| 3 | C, G (C, G) | Histone H2A type 1 | polymer | 129 | 13990.3 | 2 | UniProt (P0C0S8) | Homo sapiens (human) | H2A.1,Histone H2A/ptl |
| 4 | D, H (D, H) | Histone H2B type 1-C/E/F/G/I | polymer | 125 | 13806.0 | 2 | UniProt (P62807) | Homo sapiens (human) | Histone H2B.1 A,Histone H2B.a,H2B/a,Histone H2B.g,H2B/g,Histone H2B.h,H2B/h,Histone H2B.k,H2B/k,Histone H2B.l,H2B/l |
| 5 | I (I) | 601 I strand (non-damaged strand) | polymer | 147 | 45138.8 | 1 | synthetic construct | ||
| 6 | J (J) | 601 J strand (damaged strand) | polymer | 35 | 10882.9 | 1 | synthetic construct | ||
| 7 | K (K) | 601 K strand (damaged strand) | polymer | 112 | 34762.1 | 1 | synthetic construct | ||
| 8 | L (L) | Bifunctional polynucleotide phosphatase/kinase | polymer | 528 | 57989.1 | 1 | UniProt (Q96T60) | Homo sapiens (human) | DNA 5'-kinase/3'-phosphatase,Polynucleotide kinase-3'-phosphatase |
Sequence modifications
A, E: 1 - 135 (UniProt: Q71DI3)
L: 2 - 521 (UniProt: Q96T60)
| PDB | External Database | Details |
|---|---|---|
| Ala 110 | Cys 111 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -6 | - | initiating methionine |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| Gly 1 | - | expression tag |
| Ala 171 | Asp 171 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 257407.8 | |
| All* | Total formula weight | 257407.8 |
