37UU

Cryo-EM structure of human Fibroblast Activation Protein alpha in complex with F7 VHH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (C, D)	F7 VHH-human IgG1 Fc fusion protein	polymer	358	39228.1	2	UniProt (P0DOX5)	Camelidae
2	B, D (A, B)	Fibroblast activation protein alpha	polymer	753	87021.7	2	UniProt (Q12884)	Homo sapiens (human)
3	E, F, G, H, I... (E, F, G, H, I...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	10	In PDB
4	O, P (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG) PubChem (24139)

Sequence modifications

C, D: 1 - 126 (PDB: 37UU)
C, D: 127 - 358 (UniProt: P0DOX5)

PDB	External Database	Details
Ser 131	Cys 222	conflict

A, B: 26 - 760 (UniProt: Q12884)

PDB	External Database	Details
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
Gly 18	-	expression tag
Gly 19	-	expression tag
Gly 20	-	expression tag
Ser 21	-	expression tag
Gly 22	-	expression tag
Gly 23	-	expression tag
Gly 24	-	expression tag
Ser 25	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	252499.7
Branched	Number of molecules	10
	Total formula weight	4244.0
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	257186.1

*Water molecules are not included.