37US

Cryo-EM structure of human Fibroblast Activation Protein alpha in complex with H4 VNAR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Fibroblast activation protein alpha	polymer	753	87021.7	2	UniProt (Q12884)	Homo sapiens (human)
2	B, D (C, D)	H4 variable new antigen receptor-human IgG Fc fusion protein	polymer	346	38318.9	2	UniProt (Q8JGJ1) UniProt (P0DOX5)	Ginglymostoma cirratum (nurse shark)
3	E, F, G, H, I... (E, F, G, H, I...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	6	In PDB
4	K, L, M, N, O... (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG) PubChem (24139)

Sequence modifications

A, B: 26 - 760 (UniProt: Q12884)

PDB	External Database	Details
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
Gly 18	-	expression tag
Gly 19	-	expression tag
Gly 20	-	expression tag
Ser 21	-	expression tag
Gly 22	-	expression tag
Gly 23	-	expression tag
Gly 24	-	expression tag
Ser 25	-	expression tag

C, D: 1 - 109 (UniProt: Q8JGJ1)

PDB	External Database	Details
Val 87	Ser 98	conflict
Ala 88	Trp 99	conflict
Gly 89	Tyr 100	conflict
Met 90	Gly 101	conflict
Ser 91	Tyr 102	conflict
Pro 92	Asp 103	conflict
Leu 94	Ala 105	conflict
Ser 95	Glu 106	conflict
Trp 96	Leu 107	conflict
Gly 97	-	insertion
Asp 102	Gly 112	conflict
Ala 105	Val 115	conflict
Gly 110	-	linker
Pro 111	-	linker
Gly 112	-	linker
Gly 113	-	linker
Pro 114	-	linker

C, D: 115 - 346 (UniProt: P0DOX5)

PDB	External Database	Details
Ser 119	Cys 222	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	250681.2
Branched	Number of molecules	6
	Total formula weight	2546.4
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	254554.8

*Water molecules are not included.