37OP

Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing RNA:DNA hybrid insert sequence GAAGAGAAGCAC

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	Replication initiation protein	polymer	263	30749.1	1	UniProt (P03856)	Escherichia coli	Protein E,Protein rep,Protein F4, Replication Initiator protein RepE54
2	B (B)	DNA (5'-D(*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*G)-3')	polymer	21	6383.1	1		Escherichia coli
3	C (E)	DNA (33-MER)	polymer	33	10143.5	1		Escherichia coli
4	D (D)	RNA (5'-R(*GP*AP*AP*GP*AP*GP*AP*AP*GP*CP*AP*C)-3')	polymer	12	3921.5	1		Escherichia coli
5	E (C)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)

Sequence modifications

C: 1 - 251 (UniProt: P03856)

PDB	External Database	Details
Met -11	-	initiating methionine
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Pro 118	Arg 118	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	51197.2
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	51221.5

*Water molecules are not included.