37ON

Crystal structure of the Mucin-type O-glycosyltransferase PGANT5A-4M (A430T, L434N, A437N, S456Y)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Polypeptide N-acetylgalactosaminyltransferase 5	polymer	555	63482.1	4	UniProt (Q6WV17) Pfam (PF00535) Pfam (PF00652)	Drosophila melanogaster (fruit fly)	pp-GaNTase 5,Protein-UDP acetylgalactosaminyltransferase 5,UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 5
2	E (E)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
3	F, JA, Q, Z (A, D, B, C)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	4	Chemie (UDP)
4	BA, CA, DA, EA, G... (C, A, D, B)	GLYCEROL	non-polymer		92.1	13	Chemie (GOL) PubChem (753)
5	AA, H (C, A)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4) PubChem (8200)
6	HA, J, KA, O, P... (C, A, D, B)	1,2-ETHANEDIOL	non-polymer		62.1	7	Chemie (EDO)
7	GA, MA, N, NA, X (C, D, A, B)	MANGANESE (II) ION	non-polymer		54.9	5	Chemie (MN) PubChem (27854)
8	FA, LA, R, W (C, D, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG) PubChem (24139)
9	OA, PA, QA, RA (A, B, C, D)	water	water		18.0	26	Chemie (HOH)

Sequence modifications

A, B, C, D: 77 - 630 (UniProt: Q6WV17)

PDB	External Database	Details
Gly 76	-	expression tag
Thr 430	Ala 430	engineered mutation
Asn 434	Leu 434	engineered mutation
Asn 437	Ala 437	engineered mutation
Tyr 456	Ser 456	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	253928.6
Branched	Number of molecules	1
	Total formula weight	748.7
Non-Polymers*	Number of molecules	35
	Total formula weight	4796.3
All*	Total formula weight	259473.6

*Water molecules are not included.