36RP

Cryo-EM structure of Sulfolobus acidocaldarius RNA polymerase without stalk (no Rpo4/Rpo7)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA-directed RNA polymerase subunit Rpo1N	polymer	880	99929.1	1	UniProt (P11512)	Sulfolobus acidocaldarius DSM 639	DNA-directed RNA polymerase subunit A,DNA-directed RNA polymerase subunit A',Rpo1'
2	B (B)	DNA-directed RNA polymerase subunit Rpo2	polymer	1126	126647.1	1	UniProt (P11513)	Sulfolobus acidocaldarius DSM 639	DNA-directed RNA polymerase subunit B
3	C (C)	DNA-directed RNA polymerase subunit Rpo1C	polymer	393	44507.4	1	UniProt (P11514)	Sulfolobus acidocaldarius DSM 639	DNA-directed RNA polymerase subunit A'',DNA-directed RNA polymerase subunit C,Rpo1''
4	D (G)	DNA-directed RNA polymerase subunit Rpo8	polymer	124	14463.9	1	UniProt (Q4JAY4)	Sulfolobus acidocaldarius DSM 639	DNA-directed RNA polymerase,subunit G
5	E (H)	DNA-directed RNA polymerase subunit Rpo5	polymer	84	9477.1	1	UniProt (P11521)	Sulfolobus acidocaldarius DSM 639	DNA-directed RNA polymerase subunit H
6	F (K)	DNA-directed RNA polymerase subunit Rpo6	polymer	89	10252.0	1	UniProt (P39463)	Sulfolobus acidocaldarius DSM 639	DNA-directed RNA polymerase subunit K
7	G (L)	DNA-directed RNA polymerase subunit Rpo11	polymer	90	10061.8	1	UniProt (P46217)	Sulfolobus acidocaldarius DSM 639	DNA-directed RNA polymerase subunit L
8	H (N)	DNA-directed RNA polymerase subunit Rpo10	polymer	66	7673.1	1	UniProt (P39472)	Sulfolobus acidocaldarius DSM 639	DNA-directed RNA polymerase subunit N
9	I (Y)	DNA-directed RNA polymerase subunit Rpo13	polymer	105	12331.7	1	UniProt (Q4JAJ6)	Sulfolobus acidocaldarius DSM 639	DNA-directed RNA polymerase subunit F
10	J (D)	DNA-directed RNA polymerase subunit Rpo3	polymer	264	29858.8	1	UniProt (P39471)	Sulfolobus acidocaldarius DSM 639	DNA-directed RNA polymerase subunit D
11	K (P)	DNA-directed RNA polymerase subunit Rpo12	polymer	48	5662.9	1	UniProt (Q4JAE8)	Sulfolobus acidocaldarius DSM 639	DNA-directed RNA polymerase subunit P
12	L, M, N, O, Q (A, B, N, P)	ZINC ION	non-polymer		65.4	5	Chemie (ZN) PubChem (32051)
13	P (D)	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)

Sequence modifications

B: 1 - 1126 (UniProt: P11513)

PDB	External Database	Details
Ile 772	Val 772	conflict

G: 4 - 124 (UniProt: Q4JAY4)

PDB	External Database	Details
Met 1	-	initiating methionine
Lys 2	-	expression tag
Glu 3	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	370864.7
Non-Polymers*	Number of molecules	6
	Total formula weight	678.7
All*	Total formula weight	371543.4

*Water molecules are not included.