36FU

Crystal Structure of a Tower-SLAC, a Laccase from Streptomyces coelicolor with a Tower Loop Mutation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Copper oxidase	polymer	355	38393.5	1	UniProt (Q9XAL8) Pfam (PF07732) Pfam (PF07731)	Streptomyces coelicolor
2	B, C, D, E (A)	COPPER (II) ION	non-polymer		63.5	4	Chemie (CU) PubChem (27099)
3	F (A)	HYDROXIDE ION	non-polymer		17.0	1	Chemie (OH) PubChem (961)
4	G, H, I, K (A)	GLYCINE	non-polymer		75.1	4	Chemie (GLY) PubChem (750)
5	J, L (A)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4) PubChem (8200)
6	M (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL) PubChem (312)
7	N (A)	water	water		18.0	124	Chemie (HOH)

Sequence modifications

A: 1 - 347 (UniProt: Q9XAL8)

PDB	External Database	Details
Phe 197	-	insertion
Asn 198	-	insertion
Gly 199	-	insertion
Gly 200	-	insertion
Asn 201	Asp 197	engineered mutation
Phe 202	Met 198	engineered mutation
Leu 348	-	expression tag
Glu 349	-	expression tag
His 350	-	expression tag
His 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag
His 354	-	expression tag
His 355	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	38393.5
Non-Polymers*	Number of molecules	12
	Total formula weight	995.4
All*	Total formula weight	39388.9

*Water molecules are not included.