36DK

HIV-1 reverse transcriptase (F227C) in complex with DNA Aptamer and Incorporated dATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Reverse transcriptase/ribonuclease H	polymer	562	64708.2	1	UniProt (P04585) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075)	Human immunodeficiency virus 1	Exoribonuclease H,p66 RT
2	B (B)	p51 RT	polymer	455	53201.0	1	UniProt (P04585) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815)	Human immunodeficiency virus 1
3	C (F)	DNAdG (38-MER)	polymer	39	12061.7	1		synthetic construct
4	D (A)	PYROPHOSPHATE 2-	non-polymer		176.0	1	Chemie (POP)
5	E, F (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)
6	G, H, I (A, B, F)	water	water		18.0	8	Chemie (HOH)

Sequence modifications

A: 1 - 560 (UniProt: P04585)

PDB	External Database	Details
Met -1	-	initiating methionine
Val 0	-	expression tag
Cys 227	Phe 814	engineered mutation
Cys 258	Gln 845	engineered mutation
Ser 280	Cys 867	engineered mutation

B: 1 - 440 (UniProt: P04585)

PDB	External Database	Details
Met -14	-	initiating methionine
Ala -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ser 280	Cys 867	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	129971.0
Non-Polymers*	Number of molecules	3
	Total formula weight	224.6
All*	Total formula weight	130195.5

*Water molecules are not included.