36AP

Mycobacterium tuberculosis DNA Gyrase catalytic core in complex with DNA and indole-3-acetyl-L-aspartic acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	DNA gyrase subunit A	polymer	500	55928.3	2	UniProt (P9WG47) Pfam (PF00521)	Mycobacterium tuberculosis	Type IIA topoisomerase subunit GyrA
2	B, D (B, D)	DNA gyrase subunit B	polymer	252	28185.3	2	UniProt (P9WG45) Pfam (PF01751) Pfam (PF00986)	Mycobacterium tuberculosis
3	E (E)	DNA (5'-D(P*AP*AP*TP*GP*CP*AP*CP*GP*TP*AP*T)-3')	polymer	11	3357.2	1		synthetic construct
4	F (H)	DNA (5'-D(P*AP*TP*AP*CP*GP*TP*GP*CP*AP*T)-3')	polymer	10	3044.0	1		synthetic construct
5	G (F)	DNA (5'-D(P*TP*TP*CP*AP*TP*GP*AP*C)-3')	polymer	8	2401.6	1		synthetic construct
6	H (G)	DNA (5'-D(P*GP*GP*TP*CP*AP*TP*GP*AP*A)-3')	polymer	9	2779.8	1		synthetic construct
7	I, K, L, N (A, B, C, D)	N-[1H-INDOL-3-YL-ACETYL]ASPARTIC ACID	non-polymer		290.3	4	Chemie (IAD)
8	J, M (B, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)

Sequence modifications

A, C: 2 - 500 (UniProt: P9WG47)

PDB	External Database	Details
Ile 501	-	expression tag

B, D: 426 - 675 (UniProt: P9WG45)

PDB	External Database	Details
Asn 424	-	expression tag
Ala 425	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	179809.9
Non-Polymers*	Number of molecules	6
	Total formula weight	1209.7
All*	Total formula weight	181019.6

*Water molecules are not included.