35VQ

Q108K:K40L:T51V:T53C:R58Y:Y19W:Q38L:Q4A:T29L mutant of hCRBPII bound to FR1V in the dark at pH 6.0

Replaces:  9DE1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	Retinol-binding protein 2	polymer	133	15551.6	2	UniProt (P50120) Pfam (PF00061)	Homo sapiens (human)	Cellular retinol-binding protein II,CRBP-II
2	C, H (B, A)	(4aP)-N,N-diethyl-9,9-dimethyl-7-[(1E)-prop-1-en-1-yl]-9H-fluoren-2-amine	non-polymer		305.5	2	Chemie (ZFJ) PubChem (162394540)
3	D, I (B, A)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT) PubChem (175)
4	E, F (B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL) PubChem (753)
5	G, J (B, A)	MALONATE ION	non-polymer		102.0	2	Chemie (MLI) PubChem (9084)
6	K, L (B, A)	water	water		18.0	214	Chemie (HOH)

Sequence modifications

B, A: 1 - 133 (UniProt: P50120)

PDB	External Database	Details
Ala 4	Gln 5	engineered mutation
Trp 19	Tyr 20	engineered mutation
Leu 29	Thr 30	engineered mutation
Leu 38	Gln 39	engineered mutation
Leu 40	Lys 41	engineered mutation
Val 51	Thr 52	engineered mutation
Cys 53	Thr 54	engineered mutation
Tyr 58	Arg 59	engineered mutation
Lys 108	Gln 109	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	31103.2
Non-Polymers*	Number of molecules	8
	Total formula weight	1117.3
All*	Total formula weight	32220.5

*Water molecules are not included.