33AG

Cryo-EM structure of the L. pneumophila Ceg14 effector in complex with AnkJ

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Maltose/maltodextrin-binding periplasmic protein,Dot/Icm secretion system substrate	polymer	1060	119080.6	1	UniProt (P0AEX9) UniProt (Q5ZYD5)	Legionella pneumophila	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP
2	B (C)	Ankyrin repeat-containing protein	polymer	306	34558.2	1	UniProt (Q5ZYD6)	Legionella pneumophila

Sequence modifications

B: -372 - -7 (UniProt: P0AEX9)

PDB	External Database	Details
Met -393	-	initiating methionine
Gly -392	-	expression tag
Ser -391	-	expression tag
Ser -390	-	expression tag
His -389	-	expression tag
His -388	-	expression tag
His -387	-	expression tag
His -386	-	expression tag
His -385	-	expression tag
His -384	-	expression tag
Ser -383	-	expression tag
Ser -382	-	expression tag
Gly -381	-	expression tag
Leu -380	-	expression tag
Val -379	-	expression tag
Pro -378	-	expression tag
Arg -377	-	expression tag
Gly -376	-	expression tag
Ser -375	-	expression tag
His -374	-	expression tag
Met -373	-	expression tag
Val -61	Ala 338	conflict
Asn -6	-	linker
Ser -5	-	linker
Gly -4	-	linker
Ser -3	-	linker
Ser -2	-	linker
Gly -1	-	linker
Ser 0	-	linker
Ser 1	-	linker
Gln 2	-	linker

B: 3 - 666 (UniProt: Q5ZYD5)
C: 2 - 269 (UniProt: Q5ZYD6)

PDB	External Database	Details
Met -36	-	initiating methionine
Gly -35	-	expression tag
Ser -34	-	expression tag
Ser -33	-	expression tag
His -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
Ser -26	-	expression tag
Ser -25	-	expression tag
Gly -24	-	expression tag
Leu -23	-	expression tag
Val -22	-	expression tag
Pro -21	-	expression tag
Arg -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Met -16	-	expression tag
Ala -15	-	expression tag
Ser -14	-	expression tag
Met -13	-	expression tag
Thr -12	-	expression tag
Gly -11	-	expression tag
Gly -10	-	expression tag
Gln -9	-	expression tag
Gln -8	-	expression tag
Met -7	-	expression tag
Gly -6	-	expression tag
Arg -5	-	expression tag
Asp -4	-	expression tag
Pro -3	-	expression tag
Asn -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	153638.8
All*	Total formula weight	153638.8

*Water molecules are not included.