32ZR
B. subtilis RNA Polymerase delta omega variant with SigA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (E) | DNA-directed RNA polymerase subunit epsilon | polymer | 69 | 8228.4 | 1 | UniProt (A0A9W5LJC9) | Bacillus subtilis | RNAP epsilon subunit,RNA polymerase epsilon subunit,Transcriptase subunit epsilon |
| 2 | B, C (U, V) | DNA-directed RNA polymerase subunit alpha | polymer | 314 | 34842.4 | 2 | UniProt (P20429) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Bacillus subtilis | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 3 | D (X) | DNA-directed RNA polymerase subunit beta | polymer | 1193 | 133847.9 | 1 | UniProt (P37870) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Bacillus subtilis | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 4 | E (Y) | DNA-directed RNA polymerase subunit beta' | polymer | 1209 | 135826.4 | 1 | UniProt (P37871) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Bacillus subtilis | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 5 | F (R) | RNA | polymer | 5 | 1547.0 | 1 | Bacillus subtilis | ||
| 6 | G (G) | RNA polymerase sigma factor SigA | polymer | 371 | 43007.4 | 1 | UniProt (P06224) Pfam (PF03979) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Bacillus subtilis | Sigma-43,Sigma-A |
| 7 | H (K) | SigA controlled promoter Pveg | polymer | 60 | 18370.9 | 1 | GenBank (2731940075) | Bacillus subtilis | |
| 8 | I (L) | SigA controlled promoter Pveg | polymer | 63 | 19607.6 | 1 | GenBank (1208653917) | Bacillus subtilis | |
| 9 | J (D) | DNA-directed RNA polymerase subunit delta | polymer | 173 | 20417.9 | 1 | UniProt (P12464) Pfam (PF05066) | Bacillus subtilis | RNAP delta factor |
| 10 | K (Y) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 11 | L, M (Y) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) PubChem (32051) |
Sequence modifications
E: 1 - 69 (UniProt: A0A9W5LJC9)
Y: 1 - 1199 (UniProt: P37871)
K: 24 - 60 (GenBank: 2731940075)
| PDB | External Database | Details |
|---|---|---|
| Leu 42 | Phe 42 | conflict |
| PDB | External Database | Details |
|---|---|---|
| His 1200 | - | expression tag |
| His 1201 | - | expression tag |
| His 1202 | - | expression tag |
| His 1203 | - | expression tag |
| His 1204 | - | expression tag |
| His 1205 | - | expression tag |
| His 1206 | - | expression tag |
| His 1207 | - | expression tag |
| His 1208 | - | expression tag |
| His 1209 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Da 1 | - | expression tag |
| Dt 2 | - | expression tag |
| Dc 3 | - | expression tag |
| Dc 4 | - | expression tag |
| Da 5 | - | expression tag |
| Dc 6 | - | expression tag |
| Dc 7 | - | expression tag |
| Dt 8 | - | expression tag |
| Dc 9 | - | expression tag |
| Da 10 | - | expression tag |
| Dg 11 | - | expression tag |
| Da 12 | - | expression tag |
| Dt 13 | - | expression tag |
| Dg 14 | - | expression tag |
| Dt 15 | - | expression tag |
| Da 16 | - | expression tag |
| Da 17 | - | expression tag |
| Da 18 | - | expression tag |
| Dt 19 | - | expression tag |
| Da 20 | - | expression tag |
| Da 21 | - | expression tag |
| Dc 22 | - | expression tag |
| Da 23 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 450538.2 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 450693.3 |
