32ZQ

B. subtilis RNA Polymerase delta/omega variant with SigA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (E)	DNA-directed RNA polymerase subunit epsilon	polymer	69	8228.4	1	UniProt (A0A9W5LJC9)	Bacillus subtilis	RNAP epsilon subunit,RNA polymerase epsilon subunit,Transcriptase subunit epsilon
2	B, C (U, V)	DNA-directed RNA polymerase subunit alpha	polymer	314	34842.4	2	UniProt (P20429)	Bacillus subtilis	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
3	D (X)	DNA-directed RNA polymerase subunit beta	polymer	1193	133847.9	1	UniProt (P37870)	Bacillus subtilis	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
4	E (Y)	DNA-directed RNA polymerase subunit beta'	polymer	1209	135826.4	1	UniProt (P37871)	Bacillus subtilis	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
5	F (R)	RNA	polymer	5	1531.0	1		Bacillus subtilis
6	G (G)	RNA polymerase sigma factor SigA	polymer	371	43007.4	1	UniProt (P06224)	Bacillus subtilis	Sigma-43,Sigma-A
7	H (K)	SigA controlled promoter Pveg	polymer	63	19292.5	1	GenBank (2731940075)	Bacillus subtilis
8	I (L)	SigA controlled promoter Pveg	polymer	63	19607.6	1	GenBank (1208653917)	Bacillus subtilis
9	J (Y)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)
10	K, L (Y)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)

Sequence modifications

E: 1 - 69 (UniProt: A0A9W5LJC9)

PDB	External Database	Details
Leu 42	Phe 42	conflict

Y: 1 - 1199 (UniProt: P37871)

PDB	External Database	Details
His 1200	-	expression tag
His 1201	-	expression tag
His 1202	-	expression tag
His 1203	-	expression tag
His 1204	-	expression tag
His 1205	-	expression tag
His 1206	-	expression tag
His 1207	-	expression tag
His 1208	-	expression tag
His 1209	-	expression tag

K: 24 - 63 (GenBank: 2731940075)

PDB	External Database	Details
Da 1	-	expression tag
Dt 2	-	expression tag
Dc 3	-	expression tag
Dc 4	-	expression tag
Da 5	-	expression tag
Dc 6	-	expression tag
Dc 7	-	expression tag
Dt 8	-	expression tag
Dc 9	-	expression tag
Da 10	-	expression tag
Dg 11	-	expression tag
Da 12	-	expression tag
Dt 13	-	expression tag
Dg 14	-	expression tag
Dt 15	-	expression tag
Da 16	-	expression tag
Da 17	-	expression tag
Da 18	-	expression tag
Dt 19	-	expression tag
Da 20	-	expression tag
Da 21	-	expression tag
Dc 22	-	expression tag
Da 23	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	431025.9
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	431181.0

*Water molecules are not included.