32WJ
Structure of TRIM8 RING domain in complex with UBE2N~Ub conjugate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, G, H (I, J, B, C) | E3 ubiquitin-protein ligase TRIM8 | polymer | 83 | 9358.8 | 4 | UniProt (Q9BZR9) Pfam (PF13445) | Homo sapiens (human) | Glioblastoma-expressed RING finger protein,RING finger protein 27,RING-type E3 ubiquitin transferase TRIM8,Tripartite motif-containing protein 8 |
| 2 | C, D, I, J (K, L, D, E) | Ubiquitin-conjugating enzyme E2 N | polymer | 154 | 17279.9 | 4 | UniProt (P61088) Pfam (PF00179) | Homo sapiens (human) | Bendless-like ubiquitin-conjugating enzyme,E2 ubiquitin-conjugating enzyme N,Ubc13,UbcH13,Ubiquitin carrier protein N,Ubiquitin-protein ligase N |
| 3 | E, F, K, L (M, N, F, H) | Ubiquitin | polymer | 76 | 8576.8 | 4 | UniProt (P0CG48) Pfam (PF00240) | Homo sapiens (human) | Polyubiquitin-C |
| 4 | M, N, O, P, Q... (I, J, B, C) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) PubChem (32051) | |||
| 5 | AA, BA, CA, DA, EA... (B, C, D, E, F...) | water | water | 18.0 | 178 | Chemie (HOH) |
Sequence modifications
I, J, B, C: 2 - 81 (UniProt: Q9BZR9)
K, L, D, E: 1 - 152 (UniProt: P61088)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Pro 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Pro 0 | - | expression tag |
| Lys 87 | Cys 87 | engineered mutation |
| Ala 92 | Lys 92 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 140862.1 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 523.3 | |
| All* | Total formula weight | 141385.4 |
